Telomerase enhances osteogenic ifferentiation of sheep bone marrow mesenchymal stem cells (BMSCs) by up-regulating PI3K/Akt pathway in vitro

Telomerase reverse transcriptase (TERT) vectors were transfected into bone marrow mesen- chymal stem cells (BMSCs) which then cultured and selected to establish TERT-BMSC cell lines whilst sequencing BMSCs TERT-BMSCs via transcriptome in this study explore their regulatory mechanism effect on osteogenic differentiation after TERT ectopic expres- sion sheep BMSCs. After analysing differential genes, PI3K/Akt signalling pathway related was investigated. Western blot used before applying the inhibitor LY294002 detect protein expression levels of AKT p-AKT. On twenty-first day differentiation, RT-qPCR mRNA RUNX2 OPN alizarin red staining utilised analyse calcium salt deposition. Results showed that pro- tein p-AKT significantly up-regulated, increased deposition TERT. LY294002, down-regulated, reduced reduced. These results confirmed stable integration exogenous gene promote BMSC osteoblasts, may be mediated by pathway.